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p38  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p38
    P38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 30317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p38+mapk+antibody/p38+MAPK+Antibody/pmc13019078-153-13-23
    Average 99 stars, based on 30317 article reviews
    p38 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    RNA Extraction:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    Isolation:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    other:

    Article Title: Probiotics-enhanced kynurenic acid mitigates cisplatin-induced nephrotoxicity in mice
    Article Snippet: p38 MAPK Antibody , CST , Cat# 9212, RRID: AB_330713.

    Recombinant:

    Article Title: Phosphoglycerate dehydrogenase-mediated serine reprogramming aggravates macrophage hyperinflammation in murine Pseudomonas aeruginosa pneumonia
    Article Snippet: .. Primary antibodies used in this study include Phgdh (Proteintech, Cat. No. 14719-1-AP, 1:1000), Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (CST, Cat. No. 4370 T, 1:1000), p44/42 MAPK (ERK1/2) Antibody (CST, Cat. No. 9102S, 1:1000), Phospho-p38 MAPK (Thr180/Tyr182) Antibody (CST, Cat. No. 9211S, 1:1000), p38 MAPK Antibody (CST, Cat. No. 9212S, 1:1000), p-JNK (Santa, Cat. No. sc-6254, 1:500), JNK (Santa, Cat. No. sc-7345, 1:500), MKP-2/DUSP4 (Proteintech, Cat. No. 14719-1-AP, 1:1000), DUSP2 (Proteintech, Cat. No. 27327-1-AP, 1:1000), Histone H3 (tri methyl K27) (Abcam, Cat. No. ab6002, 1:1000), Histone H3 (Abcam, Cat. No. ab201456, 1:1000), iNOS (Proteintech, Cat. No. 18985-1-AP, 1:2500), Arginase-1 (Proteintech, Cat. No. 16001-1-AP, 1:1000), Recombinant Anti-GAPDH antibody (HRP Conjugated) (Servicebio, Cat. No. ZB15004-HRP-100, 1:2500), β-Actin Antibody (CST, Cat. No. 4967S, 1:1000), and HSP90 alpha/beta (Santa, Cat. No. sc-13119, 1:1000). .. Primary antibody binding was detected using anti-mouse IgG, HRP-linked Antibody (CST, Cat. No. 7076S, 1:5000) or anti-rabbit IgG, HRP-linked Antibody (CST, Cat. No. 7074S, 1:5000) and visualized by chemiluminescence.

    Article Title: Phosphoglycerate dehydrogenase-mediated serine reprogramming aggravates macrophage hyperinflammation in murine Pseudomonas aeruginosa pneumonia.
    Article Snippet: .. Primary antibodies used in this study include Phgdh (Proteintech, Cat. No. 14719-1-AP, 1:1000), Phosphop44/42 MAPK (ERK1/2) (Thr202/Tyr204) (CST, Cat. No. 4370T, 1:1000), p44/42 MAPK (ERK1/2) Antibody (CST, Cat. No. 9102S, 1:1000), Phospho-p38 MAPK (Thr180/Tyr182) Antibody (CST, Cat. No. 9211S, 1:1000), p38 MAPK Antibody (CST, Cat. No. 9212S, 1:1000), p-JNK (Santa, Cat. No. sc-6254, 1:500), JNK (Santa, Cat. No. sc-7345, 1:500), MKP-2/DUSP4 (Proteintech, Cat. No. 14719-1-AP, 1:1000), DUSP2 (Proteintech, Cat. No. 27327-1-AP, 1:1000), Histone H3 (tri methyl K27) (Abcam, Cat. No. ab6002, 1:1000), Histone H3 (Abcam, Cat. No. ab201456, 1:1000), iNOS (Proteintech, Cat. No. 18985-1- AP, 1:2500), Arginase-1 (Proteintech, Cat. No. 16001-1-AP, 1:1000), Recombinant Anti-GAPDH antibody (HRP Conjugated) (Servicebio, Cat. No. ZB15004-HRP-100, 1:2500), β-Actin Antibody (CST, Cat. No. 4967S, 1:1000), and HSP90 alpha/beta (Santa, Cat. No. sc13119, 1:1000). .. Primary antibody binding was detected using antimouse IgG, HRP-linked Antibody (CST, Cat. No. 7076S, 1:5000) or antirabbit IgG, HRP-linked Antibody (CST, Cat. No. 7074S, 1:5000) and visualized by chemiluminescence.

    Injection:

    Article Title: Mechanistic Insights into Toutongping Granule for Migraine: A Multi-Pathway Intervention Study in Rats
    Article Snippet: .. Nitroglycerin injection (Shandong Shenglu Pharmaceutical Co., Ltd, batch number: 2303181); TTP (The Second Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Lot No.: 230726); Rimegepant (Nurtec@, Lot No.: 5231395PA); ELISA Kits for Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-6, and CGRP (Shanghai Yuanju Biotechnology Center, Lot No.: 202405); SGExcel FastSYBR Master Premix (Sangon Biotech@, batch number: JC19KA2350); miRNA fluorescent quantitative PCR kit (staining method) (Sangon Biotech@, lot number: K506KA 4049); microRNA Extraction Kit (Sangon Biotech@, lot number: J719KA 0200); CGRP antibody (Proteintech@, Lot No. 00011200); RAMP1 Antibody (Proteintech@, 00116158); AKT Antibody (Abways, AY0420); mTOR Antibody (Abways, CY5306); p38 MAPK Antibody (PTG, 14064-1-AP); p-STAT1 Antibody (Abways, CY6498); NRF2 Antibody (PTG, 16396-1-AP); GPX4 Antibody (PTG, 67763-1-IG); PKA antibody (Cell Signaling Technology, 47825); cAMP antibody (ABclonal, A1640); p-CREB antibody (Cell Signaling Technology, 9198 T). .. Eighty SPF healthy male Sprague-Dawley (SD) rats, weighing 180-220 g and aged 3 to 4 months, were obtained from Liaoning Changsheng Biotechnology Co., Ltd (license number: SCXK (Liao) 2020-0001).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mechanistic Insights into Toutongping Granule for Migraine: A Multi-Pathway Intervention Study in Rats
    Article Snippet: .. Nitroglycerin injection (Shandong Shenglu Pharmaceutical Co., Ltd, batch number: 2303181); TTP (The Second Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Lot No.: 230726); Rimegepant (Nurtec@, Lot No.: 5231395PA); ELISA Kits for Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-6, and CGRP (Shanghai Yuanju Biotechnology Center, Lot No.: 202405); SGExcel FastSYBR Master Premix (Sangon Biotech@, batch number: JC19KA2350); miRNA fluorescent quantitative PCR kit (staining method) (Sangon Biotech@, lot number: K506KA 4049); microRNA Extraction Kit (Sangon Biotech@, lot number: J719KA 0200); CGRP antibody (Proteintech@, Lot No. 00011200); RAMP1 Antibody (Proteintech@, 00116158); AKT Antibody (Abways, AY0420); mTOR Antibody (Abways, CY5306); p38 MAPK Antibody (PTG, 14064-1-AP); p-STAT1 Antibody (Abways, CY6498); NRF2 Antibody (PTG, 16396-1-AP); GPX4 Antibody (PTG, 67763-1-IG); PKA antibody (Cell Signaling Technology, 47825); cAMP antibody (ABclonal, A1640); p-CREB antibody (Cell Signaling Technology, 9198 T). .. Eighty SPF healthy male Sprague-Dawley (SD) rats, weighing 180-220 g and aged 3 to 4 months, were obtained from Liaoning Changsheng Biotechnology Co., Ltd (license number: SCXK (Liao) 2020-0001).

    Real-time Polymerase Chain Reaction:

    Article Title: Mechanistic Insights into Toutongping Granule for Migraine: A Multi-Pathway Intervention Study in Rats
    Article Snippet: .. Nitroglycerin injection (Shandong Shenglu Pharmaceutical Co., Ltd, batch number: 2303181); TTP (The Second Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Lot No.: 230726); Rimegepant (Nurtec@, Lot No.: 5231395PA); ELISA Kits for Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-6, and CGRP (Shanghai Yuanju Biotechnology Center, Lot No.: 202405); SGExcel FastSYBR Master Premix (Sangon Biotech@, batch number: JC19KA2350); miRNA fluorescent quantitative PCR kit (staining method) (Sangon Biotech@, lot number: K506KA 4049); microRNA Extraction Kit (Sangon Biotech@, lot number: J719KA 0200); CGRP antibody (Proteintech@, Lot No. 00011200); RAMP1 Antibody (Proteintech@, 00116158); AKT Antibody (Abways, AY0420); mTOR Antibody (Abways, CY5306); p38 MAPK Antibody (PTG, 14064-1-AP); p-STAT1 Antibody (Abways, CY6498); NRF2 Antibody (PTG, 16396-1-AP); GPX4 Antibody (PTG, 67763-1-IG); PKA antibody (Cell Signaling Technology, 47825); cAMP antibody (ABclonal, A1640); p-CREB antibody (Cell Signaling Technology, 9198 T). .. Eighty SPF healthy male Sprague-Dawley (SD) rats, weighing 180-220 g and aged 3 to 4 months, were obtained from Liaoning Changsheng Biotechnology Co., Ltd (license number: SCXK (Liao) 2020-0001).

    Staining:

    Article Title: Mechanistic Insights into Toutongping Granule for Migraine: A Multi-Pathway Intervention Study in Rats
    Article Snippet: .. Nitroglycerin injection (Shandong Shenglu Pharmaceutical Co., Ltd, batch number: 2303181); TTP (The Second Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Lot No.: 230726); Rimegepant (Nurtec@, Lot No.: 5231395PA); ELISA Kits for Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-6, and CGRP (Shanghai Yuanju Biotechnology Center, Lot No.: 202405); SGExcel FastSYBR Master Premix (Sangon Biotech@, batch number: JC19KA2350); miRNA fluorescent quantitative PCR kit (staining method) (Sangon Biotech@, lot number: K506KA 4049); microRNA Extraction Kit (Sangon Biotech@, lot number: J719KA 0200); CGRP antibody (Proteintech@, Lot No. 00011200); RAMP1 Antibody (Proteintech@, 00116158); AKT Antibody (Abways, AY0420); mTOR Antibody (Abways, CY5306); p38 MAPK Antibody (PTG, 14064-1-AP); p-STAT1 Antibody (Abways, CY6498); NRF2 Antibody (PTG, 16396-1-AP); GPX4 Antibody (PTG, 67763-1-IG); PKA antibody (Cell Signaling Technology, 47825); cAMP antibody (ABclonal, A1640); p-CREB antibody (Cell Signaling Technology, 9198 T). .. Eighty SPF healthy male Sprague-Dawley (SD) rats, weighing 180-220 g and aged 3 to 4 months, were obtained from Liaoning Changsheng Biotechnology Co., Ltd (license number: SCXK (Liao) 2020-0001).

    Extraction:

    Article Title: Mechanistic Insights into Toutongping Granule for Migraine: A Multi-Pathway Intervention Study in Rats
    Article Snippet: .. Nitroglycerin injection (Shandong Shenglu Pharmaceutical Co., Ltd, batch number: 2303181); TTP (The Second Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Lot No.: 230726); Rimegepant (Nurtec@, Lot No.: 5231395PA); ELISA Kits for Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-6, and CGRP (Shanghai Yuanju Biotechnology Center, Lot No.: 202405); SGExcel FastSYBR Master Premix (Sangon Biotech@, batch number: JC19KA2350); miRNA fluorescent quantitative PCR kit (staining method) (Sangon Biotech@, lot number: K506KA 4049); microRNA Extraction Kit (Sangon Biotech@, lot number: J719KA 0200); CGRP antibody (Proteintech@, Lot No. 00011200); RAMP1 Antibody (Proteintech@, 00116158); AKT Antibody (Abways, AY0420); mTOR Antibody (Abways, CY5306); p38 MAPK Antibody (PTG, 14064-1-AP); p-STAT1 Antibody (Abways, CY6498); NRF2 Antibody (PTG, 16396-1-AP); GPX4 Antibody (PTG, 67763-1-IG); PKA antibody (Cell Signaling Technology, 47825); cAMP antibody (ABclonal, A1640); p-CREB antibody (Cell Signaling Technology, 9198 T). .. Eighty SPF healthy male Sprague-Dawley (SD) rats, weighing 180-220 g and aged 3 to 4 months, were obtained from Liaoning Changsheng Biotechnology Co., Ltd (license number: SCXK (Liao) 2020-0001).



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    Image Search Results


    Mechanisms of Cdh2 gene in chondrogenesis. (a) Relative expression of Cdh2 gene through qRT-PCR. HD: higher density; LD: lower density. (b, c) Western blotting results of N-cadherin and p38 MAPK in different groups. Cdh2 KD : Cdh2 knocked down group. (d) Immunofluorescent results of cell pellets after 3 days of co-culture. Blue: DAPI; Green: ActinGreen; Red: N-cadherin. Scale bar = 200 μm. (e, f) Relative expression of Cdh2 gene and chondrogenesis-related genes ( Sox9 , Col2a1 , Acan ) through qRT-PCR. (g) Immunofluorescent results of cell pellets after 14 days of co-culture. Blue: DAPI; Green: ActinGreen; Red: N-cadherin. Scale bar = 200 μm ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

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    doi: 10.1016/j.bioactmat.2026.01.009

    Figure Lengend Snippet: Mechanisms of Cdh2 gene in chondrogenesis. (a) Relative expression of Cdh2 gene through qRT-PCR. HD: higher density; LD: lower density. (b, c) Western blotting results of N-cadherin and p38 MAPK in different groups. Cdh2 KD : Cdh2 knocked down group. (d) Immunofluorescent results of cell pellets after 3 days of co-culture. Blue: DAPI; Green: ActinGreen; Red: N-cadherin. Scale bar = 200 μm. (e, f) Relative expression of Cdh2 gene and chondrogenesis-related genes ( Sox9 , Col2a1 , Acan ) through qRT-PCR. (g) Immunofluorescent results of cell pellets after 14 days of co-culture. Blue: DAPI; Green: ActinGreen; Red: N-cadherin. Scale bar = 200 μm ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

    Article Snippet: After blocked with skimmed milk, the membranes were incubated with primary antibody at 4 °C (anti-N-cadherin (Abcam), anti-p38 MAPK (Proteintech)).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Co-Culture Assay

    Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Journal: Journal of Translational Autoimmunity

    Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach

    doi: 10.1016/j.jtauto.2025.100341

    Figure Lengend Snippet: Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Article Snippet: Membranes were blocked with 5 % non-fat milk in TBS-T and incubated overnight at 4 °C with primary antibodies against phospho-AKT (Thr308), phospho-NFκB p65 (Ser536), phospho-p38 MAPK (Thr180/Tyr182), phospho-STAT1 (Tyr701), and phospho-STAT3 (Tyr705) (Cell Signaling Technology, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Protein-Protein interactions, Expressing, Phospho-proteomics, Control

    Effects of dietary vitamin D 3 supplementation on jejunal phosphorylation levels of PKA, PKC, PI3K, p38MAPK, and ERK in broiler chickens (19 d) (Experiment 1). The control and vitamin D 3 diets contained 0 and 1000 IU/kg vitamin D 3 , respectively. (a) p-PKA/t-PKA; (b) p-PKC/t-PKC; (c) p-PI3K/t-PI3K; (d) p-p38MAPK/t-p38MAPK; (e) p-ERK/t-ERK. Protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Journal: Poultry Science

    Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens

    doi: 10.1016/j.psj.2026.106762

    Figure Lengend Snippet: Effects of dietary vitamin D 3 supplementation on jejunal phosphorylation levels of PKA, PKC, PI3K, p38MAPK, and ERK in broiler chickens (19 d) (Experiment 1). The control and vitamin D 3 diets contained 0 and 1000 IU/kg vitamin D 3 , respectively. (a) p-PKA/t-PKA; (b) p-PKC/t-PKC; (c) p-PI3K/t-PI3K; (d) p-p38MAPK/t-p38MAPK; (e) p-ERK/t-ERK. Protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

    Techniques: Phospho-proteomics, Control, Quantitative Proteomics

    Effects of the PKA inhibitor H-89 on jejunal phosphorylation levels of PKC, PI3K, p38MAPK, and ERK in broiler chickens (10–15 d) (Experiment 2). (a) p-PKC/t-PKC; (b) p-PI3K/t-PI3K; (c) p-p38MAPK/t-p38MAPK; (d) p-ERK/t-ERK. Protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Journal: Poultry Science

    Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens

    doi: 10.1016/j.psj.2026.106762

    Figure Lengend Snippet: Effects of the PKA inhibitor H-89 on jejunal phosphorylation levels of PKC, PI3K, p38MAPK, and ERK in broiler chickens (10–15 d) (Experiment 2). (a) p-PKC/t-PKC; (b) p-PI3K/t-PI3K; (c) p-p38MAPK/t-p38MAPK; (d) p-ERK/t-ERK. Protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

    Techniques: Phospho-proteomics, Quantitative Proteomics

    Effects of the PKC inhibitor staurosporine on duodenal phosphorylation levels of PKA, PI3K, p38MAPK, and ERK in broiler chickens (13 d) (Experiment 3). (a) p-PKA/t-PKA; (b) p-PI3K/t-PI3K; (c) p-p38MAPK/t-p38MAPK; (d) p-ERK/t-ERK. Protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Journal: Poultry Science

    Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens

    doi: 10.1016/j.psj.2026.106762

    Figure Lengend Snippet: Effects of the PKC inhibitor staurosporine on duodenal phosphorylation levels of PKA, PI3K, p38MAPK, and ERK in broiler chickens (13 d) (Experiment 3). (a) p-PKA/t-PKA; (b) p-PI3K/t-PI3K; (c) p-p38MAPK/t-p38MAPK; (d) p-ERK/t-ERK. Protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

    Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

    Techniques: Phospho-proteomics, Quantitative Proteomics